SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Industry standard, 96-well plates. This reader's dual monochromators for. This can increase throughput and efficiency, thus saving time and space in the lab. Please click on the links below to view the Microplate Reader Comparison Tables. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Next generation SpectraMax® absorbance microplate readers. QC tools and IQOQ services. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. VMax . 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. #C1500) Control standard endotoxin (ACC cat. Next-generation luminescence plate reader. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. It has a 340-750nm Spectral Range. 2. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 1. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. x. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . 00. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. The VersaMax reads 96-well microplates. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Gemini XPS Reader scans. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. the use of a reader calibrated with primary NIST and NMI standards. 030 fmol Fluorescence Polarization (FP). The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. MCF7 cells treated with. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. Growth medium was then replaced with fatty acid uptake solution. 0 . We would like to show you a description here but the site won’t allow us. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. and glow luminescence modes in 96- to 384-well plates. Primary considerations when choosing a microplate reader are throughput and flexibility. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. 2. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Click Add to create the plate. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. * Note: Programming the washer with a final aspirate height of 4. Anticancer drug assay was also conducted using conventional 96-well plates as controls. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. Related products. 40266. 1. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. the Gemini XPS reader. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 5 second read per plate. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. 00. 5. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. What are the dimensions of the plate? Code. The opaque walls prevent stray light from being detected from neighbouring. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Microplate Capacity. x. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. An example of dual-luciferase assay results. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Absorbance Linearity: 2. They can be read by most microplate readers. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. REF (SpectraMax. More Product Information. Suggested plates are black walled. #E010) Pyroplate 96-well microplate (ACC cat. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Click SoftMax Pro Software User Guide. Figure 2-23: Bottle holder To install the bottle holder: 1. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Multimodal plate reader: Yes: 25 mm: Yes—Max. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. We would like to show you a description here but the site won’t allow us. 5 ng per well. Published Dec 16, 2020 | Updated Dec 16, 2020. Molecular Devices has provided scientists with . To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. x. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. A = Log 10 (I 0 /I). Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. A. Three low volume 24-well bottom slides. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 96/384-well plate formats. x. 012 fmol 0. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Packed 25 plates per sleeve, 2 sleeves per case. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. SoftMax Pro GxP Software version 5. High tolerance for contaminants. 005 OD Normal and Rapid modes, 96- and 384-well plates;. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. 6-384 (96- and 384-well with pipetting) 96-well microplates. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Absorbance Plate Reader Comparison Chart. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. An industry-renowned instrument for monitoring GPCRs and ion channels. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. 45 µm PVDF. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. When the user applies a set temperature of 37°C, the top is heated to 37. Microplate Readers. Plus 384. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. 2. 2. HeLa. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. 9000-0756. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Microplate Spectrophotometers. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Optional 12-way manifold) 5032336. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. FlexIII. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. com. • Photometric range: 0. Populate the following info under Description. 6. 00 USD. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 9. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. x. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. TNF standard curve. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. p-Nitroaniline - Catalog No. 0% ± 0. . Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Measurements for every well are automatically tracked over time. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . 0 OD. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. EMax. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. 000 to 2. Some will only support one well density (e. Dimensions: 18. Molecular Devices L-Max II Luminescence Microplate Reader. Time-resolved phosphorescence. The plate reader optics are tunable for. 0200-6263. 305 reference level saturation. 1% in white 96- and < 0. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Microplate Readers include the M3, M4, M5, and M5e models. 96-well microplate compatible Well read area range: D4. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. These lab workhorses are used in drug discovery. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 1. Page 1 of 8 . Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. Four wash/rinse bottles are included and configurable for both 96- and 384. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. Instrument settings can. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Used. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. White 96-well plate Plate formats. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. 3 OD at 200 nm. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Compare specifications and request a quote from leading suppliers. #CA961) Methods. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Molecular Devices. External Dimensions without lid (mm). the Molecular Devices SPECTRAmax® 190 and 250. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. #E010) Pyroplate 96-well microplate (ACC cat. The CellTiter-Glo assay was used to measure ATP. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 5 fg per well to 0. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. Self-righting base, up to 40 degrees past center without dispenser. HeLa. 001 ABS (displayed), 0. Use standard plate unless you see your type listed. x. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Validation made easy -1) Select the correct plate and the wells that you want to measure. Can read 96 well plates (optional 384 well). OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 5in (46. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. adjust the reader. Fluorescence Microplate Reader Comparison Chart. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Cayman Chemical. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Reconstitution of. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 1 – 2. 1 fmol luciferase per well. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. • 0. $3,207. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. DynaPro ™ Plate Reader III. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. Other. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The procedures in this protocol support the 96-well reaction plate. 00 USD. 7°C (gradient heating design). A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. and the A1 corner of the plate adapter (Figure 1-2). Published Dec 16, 2020 | Updated Dec 06, 2021. 314 low lamp level warning. Endpoint, kinetic, spectrum. VMax . 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. It offers extremely low absorbance background, excellent. 3. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Click Create. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). 057, depending on lot number. Select up to 3 products. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Thermomax model provides thermal control and vertical shake, perfect for. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. This can increase throughput and efficiency, thus saving time and space in the lab. #iD5-STD). Absorbance equation. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Mix samples in microplate wells by user-programmable Automix feature. 5 µL of each sample was pipetted into a 96-well plate for PCR. 0, or. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. 1% in white 96- and < 0. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. Intuitive graphical user interface makes each assay extremely easy to set. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. Microplates, cuvettes (via adapter) Detectors. 020 fmol 0. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. An industry-renowned instrument for monitoring GPCRs and ion channels. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. These robust readers have been placed in labs from Antarctica to. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Janet got excellent results using them and they are only ~$1. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. 003 fmol 0. Plus 384. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. In addition, the iD5 reader measures TRF and FP and. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Consequently, FLIPR has been used. Users can choose to process all plates within the magazine or select a specific number. to each well. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. It also integrates with the. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Contents of wells were pipetted up and down several times to mix. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. 340 PC384. 2. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Quantitative, whole well cell confluence for every well. maximum-illumination-channels. Molecular Devices (UK) Ltd. Tristar 3 Multimode Microplate Reader. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. Contact our support team with your questions regarding Molecular Devices products and applications. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Learn more. ~ Bio-Tek ELx808 Microplate Reader $2,795. 2 OD at 200 nm; this decreased to 0. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. No. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. x. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. 96- and 384-well. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. These robust readers have been placed in labs from. 039. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. Published Dec 16,. Well scanning With well scanning, multiple points within each well can be read, providing a high. Molecular Devices SpectraMax Plus 384 Absorbance. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. validation, maintenance, and repair documentation. Instrument pricing. 500 OD ± 3. The advanced design of the Sunrise plate reader includes.